We propose that the increased capacity for CO2 uptake in the BicA(+) strain can be capitalized on by re-directing carbon flux from exopolymeric substances to other end products such as fuels or high-value chemicals. ![]() Bioz Stars score: 86/100, based on 1 PubMed citations. Metabolic analyses, including phenotyping by synchrotron-radiation Fourier transform Infrared spectromicroscopy, scanning electron microscopy, and lectin staining, suggest that the excess assimilated carbon in BicA(+) and BicA(+)(T485G) cells was directed into production of saccharide-rich exopolymeric substances. MacVector macvector 7 0 software Macvector 7 0 Software, supplied by MacVector, used in various techniques. However, the bicarbonate uptake capacity of the induced BicA(+)(T485G) was twice higher than for the wild-type strain. Introducing a point mutation in the large C-terminal cytosolic domain of the inserted BicA, at a site implicated in allosteric regulation of transport activity, resulted in a strain (BicA(+)(T485G) strain) that exhibited pronounced cell aggregation and failed to grow at 5% CO2. Primer Sequences and PCR Cycle Condition. 1) International Biotechnologies, Inc MacVector Sequence Analysis Package. When enriched with 0.5% or 5% CO2, the BicA(+) strain grew slower than the control but still showed a superior biomass production. The base composition plot of GC content was obtained using MacVector 3.5 software (Accelrys, San Diego, CA). My opinions are based on use of purchased software, freeware, or shareware. When cultured under atmospheric CO2 pressure, the strain expressing extra BicA transporters (BicA(+) strain) grew almost twice as fast and accumulated almost twice as much biomass as the control strain. Free, fast and easy way find a job of 969.000+ postings in Tarrytown, NY and other big cities in USA. Full-time, temporary, and part-time jobs. strain PCC6803, we engineered strains to contain additional inducible copies of the endogenous bicarbonate transporter BicA, an essential component of the CO2-concentrating mechanism in cyanobacteria. Search and apply for the latest R&d software engineer jobs in Tarrytown, NY. MacVector macvector 7 0 software Macvector 7 0 Software, supplied by MacVector, used in various techniques. McGill University, Department of Electrical & Computer Engineering. ![]() As a means to improve carbon uptake in the cyanobacterium Synechocystis sp. G6G Directory of Omics and Intelligent Software Software.
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